PCR Biosystems expands comprehensive range of diagnostic reagents
Launches Lyo-Ready Probe 1-Step Kit, a lyophilisable 4x RT-qPCR reagent mix designed for ultra-sensitive high-throughput detection of RNA sequences
PCR Biosystems has expanded its comprehensive range of diagnostic reagents with the launch of the Lyo-Ready Probe 1-Step Kit, a lyophilisable 4x RT-qPCR reagent mix designed for ultra-sensitive high-throughput detection of RNA sequences. The new reagents will bring the exceptional sensitivity of the company’s liquid format 1-step RT-qPCR reagents to a freeze-dryable format. As a result, simplified creation of freeze-dried multiplex assays with lower limits of detection will be possible, and the logistical and storage infrastructure required to deliver tests to the point of use will be streamlined. Diagnostic developers can thus accelerate their time to market, and a broader swathe of clinical, veterinary, food and environmental testing end-users will be able to benefit from superior nucleic acid detection.
Molecular diagnostics have traditionally relied on ‘wet’ reagents, necessitating costly temperature-controlled transport and on-site cold storage at the point of test use. Diagnostics developers are increasingly looking to use lyophilised test formats to circumvent these challenges and develop more compact, lighter, and shelf-stable assays. However, developing lyophilised diagnostics is complex — traditional reagent mixes contain lyophilisation-incompatible components such as glycerol, and concerns can often arise around test sensitivity after reagents are resuspended.
The new reagents from PCR Biosystems come already optimised for freeze-drying, meaning diagnostic developers only need to add their desired probes and primers before lyophilisation, unlocking smoother, faster test development. Importantly, the new reagents are pre-blended with a unique mix of excipients to ensure uncompromised sensitivity after resuspension — even for multiple RNA targets — making the reagents ideal for high-performance multiplex tests.
